The costs of developing groundwater in the Western Aquifer Basin vary considerably across the West Bank and Israel. One of the main reasons for this variability is the diverse hydrogeological conditions within the aquifer. Using data from recent hydrogeological investigations, an estimate of the variation of both the drilling and pumping costs was calculated and then mapped across the Upper and Lower Aquifers within the Western Aquifer Basin. These groundwater cost maps proved helpful in analyzing the impacts of hydrogeology on water supply, and also in communicating complex hydrogeological information to a broader audience. The maps clearly demonstrate that the most cost-effective area to develop groundwater is along the Green Line—the 1949 armistice boundary between Israel and the Palestinian West Bank. Any migration of this boundary eastwards will affect the cost and feasibility of developing groundwater within Palestine, making abstraction from the Upper Aquifer impracticable, and increasing the cost of developing the Lower Aquifer. Therefore, the separation wall, which is being constructed to the east of the Armistice Line in Palestinian territory, will significantly reduce the ability of the Palestinians to develop groundwater resources.